The prepared igg immunoprobe was found to have a sensitively nernst response to igg because of the antigen - antibody reaction . the change of potential from beginning to end ( the potentimetric difference , a 用戊二醛交聯(lián)法在鉑電極、銀電極上固定igg抗體,制備了可重復(fù)使用的電位型無標(biāo)記igg免疫探針。
Constructed the fusion protein expression vector of echistatin , . and identify it by dna sequencing . the vector can express echistatin fusion protein at a high level , the fusion protein molecular weight is about 16000 da in sds - page analysis , and the fusion protein consists more than 30 % of total protein , the fusion protein has specific antigen - antibody reaction with rabbit anti - echistatin polyclonal antibody . the fusion protein include : hpk5 . his + 6tag , and echistatin . echistatin can be released by cnbr cleavage 構(gòu)建了echistatin融合蛋白表達(dá)載體,經(jīng)dna測序鑒定正確后,溫控誘導(dǎo)表達(dá),獲得了高效表達(dá),表達(dá)產(chǎn)物經(jīng)505一隊(duì)ge分析,分子量為16000oa ,符合預(yù)計(jì)的融合蛋白分子量,表達(dá)產(chǎn)物. ll ’菌體總蛋白的30 %以上, westem一blotting結(jié)果顯示,該日的蛋白能夠和echistatin多克隆抗體發(fā)生特異性抗原抗體反應(yīng),表明我們成功構(gòu)建了eohistatin的高效融合表達(dá)克隆。